Head of the department – Shulyak Svitlana, PHD

Tel.: +38 (044) 243-37-55

E-mail: dia_sveta_@ukr.net

Department structure:

Laboratory ELISA-test and determination of mycotoxins

Laboratory of gas chromatography

Laboratory of Physical and Chemical Research

Liquid chromatography laboratory

Laboratory of Atomic Absorption Spectrometry

Areas of work of the department:

• Detection of 761 indicators of quality and safety of plant and animal origin products, food, feed, premixes, vitamins, water, beverages (including alcoholic) cosmetics, soils, which are accredited in accordance with the requirements of DSTU EN ISO / IEC 17025: 2019;
• Diagnosis and prevention of diseases of non-communicable etiology, hypovitaminosis, poisoning, mycotoxicosis, dermatomycoses;
• Development and implementation “Plan of the State monitoring of residues of veterinary drugs and contaminants in live animals, unprocessed food and feed”;
• Organization of interlaboratory comparative tests with PPT “VET-TEST” for chemical and toxicological indicators, as an accredited provider of proficiency testing in accordance with DSTU EN ISO / IEC 17043: 2017.
• Organizing and conducting trainings, scientific and practical seminars, advanced training, education, internships for specialists of state and other testing laboratories.

Elisa-test laboratory and determination of mycotoxins

Determination of the following indicators in food products of plant and animal origin, raw materials, feeds, feed additives, beverages, water:

• Mycotoxins: ochratoxin A (TLC, HPLC), aflatoxins B1, B2, G1, G2 (TLC, HPLC), aflatoxin M1 (HPLC), T-2 toxin (TLC, ELISA), zearalenone (TLC, ELISA, HPLC), deso (TLC, ELISA, HPLC), fumonisin (TLC, ELISA, HPLC), patulin (TLC, HPLC).
• Allergens: gliadin, histamine (HPLC).
• Stilbene: diethylstilbestrol (ELISA).
• Nitrosamines are a TLC method.
• Toxicity is a biological method on Tetrachimena periformis, rabbits, and white mice.
• Dermatomycoses – a microbiological method.
• Toxic, mold fungi – a microscopic, biological method.

Equipment: liquid chromatographs with double mass spectrometer LC / MS / MS Waters TQD, WatersXevo, liquid chromatographs with fluorescent detector Thermo.

Liquid chromatography laboratory

Laboratory activities:

Determination of residual quantities of veterinary drugs in products of animal origin:

• stilbene (diethylstilbestrol, dienestrol, hexestrol);
• synthetic steroids (17-β-estradiol, 19-nor-testosterone, boldenone;
• nitrofurans (AOZ, AMOZ, SEM, AHD);
• nitroimidazoles (metronidazole, ronidazole, dimetridazole, hydroxydimetridazole, ipronidazole, hydroxyipronidazole, hydroxymetronidazole, ternidazole);
• β-lactam antibiotics (penicillin, oxacillin, naphcillin, dicloxacillin, cloxacillin, amoxicillin, ampicillin);
• cephalosporins (ceftiofur, cefquin, cefopyrin, cefopyrazone, cefazolin, cephalonium, cephalexin);
• quinolones;
• tetracyclines;
• aminoglycosides (dihydrostreptomycin, gentamicin, kanamycin, paromomycin, spectinomycin, streptomycin, apramycin, lincomycin, neomycin);
• sulfonamide drugs;
• tylosin, erythromycin, dapsone, florfenicol, colistin, tiamulin, nystatin, anthelmintics (albendazole, fenbendazole, levamisole, mebendazole, triclambendazole, oxyclozanide, clozantel, ivermectin, avermectin);
• coccidiostats (diclazuril, salinomycin, monensin, narazin, dinitrocarbanilide, maduramycin, decoquinate, robenidine, toltrazuril);
• sedatives (chlorpromazine, acepromazine, xylazine);
• colchicine;

non-steroidal anti-inflammatory veterinary drugs (phenylbutazone, flunixin, naproxen, 5-hydroflunexin, meloxicam, diclofenac, ibuprofen, niflumic acid, carprofen, mefenamic acid, tolfenamic acid, ketoprofen, phenolphene, oxyfenub);

isoniazid.

Equipment: Liquid chromatographs with double mass spectrometer LC / MS / MS Waters TQD, Waters Premier, WatersXevo

Laboratory of Physical and Chemical Research

Laboratory activities:

Determination of the following accredited indicators in food products of plant and animal origin, raw materials, feed, grain, copper, feed additives:

• pH, acidity, active chlorine, urease activity, damaged and sprouted grains, ammonia, hydrogen sulfide, vitamins A, E, B1, B2, B5, B6, mineral and foreign impurities, technological water, ethanol, suspended solids, total hardness, total acidity , mass fraction of protein substances, mass fraction of protein, mass fraction of moisture, mass fraction of glaze, mass fraction of fat, mass fraction of ash, mass fraction of calcium, mass fraction of starch, mass fraction of meat with fat, mass fraction of sodium nitrite, mass fraction of crude protein, mass fraction of crude fiber , mass fraction of sorbic acid and its salts, mass fraction of dry matter, mass fraction of dry nonfat residue, mass fraction of titrated acids, mass fraction of phosphorus, mass fraction of chlorides, mass fraction of sodium chloride, nitrates, nitrites, peroxide, peroxidase, alkaline phosphatase activity , peroxide value and others.
• Water research: fluorides, nitrates, nitrites, ammonium, chlorides, sulfates, color, conductivity, hydrogen ion concentration, odor, oxidation, turbidity;
• Alcoholic products: strength, mass concentration of total extract, mass concentration of acids, alkalinity, mass concentration of aldehydes, mass concentration of fusel oil in terms of a mixture of isoamyl and isobutyl alcohols in a ratio of 1: 1 with salicylic aldehyde, mass concentration of fusel oil , isoamyl and isobutyl alcohols in a ratio of 3: 1: 1 with P-DMABA, mass concentration of esters, volume fraction of methyl alcohol, mass concentration of invert sugar, alcohol content, reducing sugars, total acidity, volatile acids, sulfur dioxide (free, total), methyl alcohol by weight, titrometric, photocolorimetric, spectrophotometric , fluorometric, aerometric, pycnometric and acidimetric methods.

Laboratory of Atomic Absorption Spectrometry

Laboratory activities:

Determination of the following accredited indicators in foodstuffs of plant and animal origin, raw materials, feeds, compound feeds and compound feed raw materials, premixes and vitamin supplements:

Lead, cadmium, arsenic, mercury, copper, zinc, iron, manganese, silver, aluminum, sodium, chromium, selenium, cobalt, tin, sodium, nickel by atomic absorption spectrometry with flame atomization and atomic absorption spectrometry with electrothermal atomization.

Determination of toxic elements and micro-, macroelements in water, beverages and alcoholic products: aluminum, antimony, arsenic, boron, cadmium, chromium, cobalt, copper, iron, lead, manganese, molybdenum, selenium, argentum, sodium, zinc, potassium, calcium, phosphorus by inductively coupled optical emission spectrometry.

Equipment: analytical emission spectrometer with inductively coupled plasma Analytik Jena Plasma Q 9000, atomic absorption spectrophotometers Varian 240 Z Varian 55B Thermo Solar, mercury analyzer Milestone DMA-80.

Laboratory of gas chromatography

Laboratory activities:

Definitions in food products of plant and animal origin, raw materials, feeds, feed additives, water:

• pesticides – by GC / DEZ, GC / MS, RH / MS-MS, IX / MS / MS and TLC;
• non-dioxin-like polychlorinated biphenyls (PCBs) – by GC / DEZ, GC / MS methods;
• volatile organic compounds (VOCs) – by GC / DEZ, GC / PID methods;
• polycyclic aromatic hydrocarbons (surfactants) – GC / MS method; GC / PID method;
• chlorates, perchlorates (IX / MS / MS);
• sterols – by GC / PID method;
• fatty acid methyl esters (FFA) – GC / PID method;
• triglycerides – by GC / PID method.

Equipment: Thermo “Focus GC” gas chromatograph with “DSQ II” mass spectrometric detector; Varian gas chromatograph “450-GC” with detector (DEZ); Varian gas chromatograph “CP-3800” with detectors (DEZ); Varian gas chromatograph “CP-3800” with detector (PID), liquid chromatograph Varian “Pro Star” with UV detector, liquid tandem chromato-mass spectrometer “Waters Xevo TQ-XS”, gas tandem chromato-mass spectrometer Thermo TSQ 9000.

Scientific and methodical work of the department:

• Development of National Standards of Ukraine, methodical recommendations, patents, other regulatory documentation;
• Providing methodological assistance to chemists-toxicologists of state laboratories of the State Tax Service by conducting annual all-Ukrainian seminars, conducting trainings, internships on the basis of SSILDVSE, training of chemists-toxicologists at the Institute of Postgraduate Education;
• Organization and holding of interlaboratory comparative rounds for the purpose of professional testing of chemists-toxicologists according to the VET-TEST program;
• Specialists of the department take part in rounds of professional testing both at the international level – FAPAS and at the national level;
• Carrying out of research work in the corresponding directions, postgraduate study, performance of the initiative-search theme “Development and improvement of methods of definition of medicines and toxicants at poisoning of animals and bees”.